Uji Toksisitas Ekstrak Etanol Daun Sirih Hijau (Piper betle L. Folium) Dari Kabupaten Wajo Terhadap Larva (Artemia Salina L.) Dengan Metode BSLT

Abstract

The development of traditional medicine is currently growing rapidly so that traditional medicine is often used among the community. Natural-based medicinal plants have few side effects and if used long term do not cause toxic effects on the body. Green betel leaves (Piper betle L. folium) are one of the plants used as medicine and contain compounds such as terpenoids, phenols and alkaloids which have cytotoxic capabilities against cancer cells. The BSLT test is an initial screening method used to test the toxicity of an extract or natural compound. The aim of this research was to determine the LC50 value of 96% ethanol extract of green betel leaves (Piper betle L. folium) from Wajo Regency based on the results of toxicity tests on larvae (Artemia salina L.) using the BSLT method. Green betel leaf extract (Piper betle L. folium) was obtained by maceration using 96% ethanol solvent. This test uses several concentrations, namely 750 ppm, 500 ppm, 250 ppm, 100 ppm, and 10 ppm which are given to larvae (Artemia salina L.) for 24 hours and then calculate the LC50 value using probit analysis. The lower the LC50 value, the higher the level of toxicity of a compound. The LC50 value obtained was 26.3 ppm, this shows that the 96% ethanol extract of green betel leaves (Piper betle L. folium) has a very toxic effect on larvae (Artemia salina L.).