Penetapan Kadar Total Fenol Ekstrak Propolis yang Menggunakan Pelarut Eutektik Dalam Kombinasi Asam Laktat, Glukosa, dan Air.

Abstract

Propolis is a resinous substance collected by bees to construct and protect their hives. This resin-based material has been used for centuries as a traditional medicine to heal wounds. The aim of this research is to determine the presence of phenolic compounds in propolis extract using Deep Eutectic Solvent (DES) as the solvent and to determine the total phenolic content in the propolis extract using a combination of DES composed of lactic acid, glucose, and water. The study was conducted experimentally by extracting propolis and performing qualitative analysis using the Folin-Ciocalteu reagent, followed by quantitative analysis using UV-Vis spectrophotometry. The research results showed positive indications of phenolic compounds, indicated by a color change to blue, with a spectrophotometric UV-Vis measurement revealing a phenol content of 0.7180%.